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Part:BBa_K4193026:Experience

Designed by: Xinchen Yue   Group: iGEM22_OUC-China   (2022-10-08)


ATP is catalyzed by AtlPt4 to form quorum-sensing molecule IP. When IP accumulates to a certain extent in the environment, it can bind with AtCRE1 to phosphorylate downstream Ypd1, and transmit phosphate signal to Skn7, so that Skn7 can induce the expression of SSRE promoter

Applications of BBa_K4193026

Different concentrations of IP can increase the expression of SSRE promoter. After rough statistical analysis, the expression intensity of SSRE promoter was significantly correlated with IP concentration.

Figure1. Effect of different concentrations of IP on SSRE promoter induction.

To get a great insight for the protein in designed signal pathway, we used dry lab to make up for the defect we could not characterize the behavior of these proteins, such as AtCRE1_P, Ypd1_P and Skn7_P interacting with different subtypes of SSRE promoter.

Figure2.The behavior of proteins in designed signal pathway

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